Y-27632 dihydrochloride (2024)

1. Validity

The present general terms of sale and delivery apply to all business of VWR International GmbH, i.e. particularly to industrial chemicals, reagents and diagnostic products as well as general laboratory supplies including equipment for the use of these products and consulting. By placing an order the customer irrevocably accepts the present conditions whose modification requires our written consent.

If a business has been concluded on the basis of the present General Terms and Conditions of Sale and Delivery, these will apply also to further business, even if no reference is made to them.

If we act as agents of other companies, particularly of Merck KgaA, Darmstadt, the General Conditions of this company shall apply to the procured business (the so-called extended retention of title with regard to claims resulting from the sale of goods which remain the property of VWR until fully paid is, in view of the Austrian laws, considered as not agreed upon for deliveries to Austria)

We do not recognize the general conditions of our customers, even if we do not explicitly reject them in a specific case.

2. Offers

Our offers and list prices are subject to alteration. Orders by our customers, even when based on our offers, shall become binding only when confirmed in writing by us or when delivered.

3. Cancellation / Return of Goods

Due to rapid delivery basically no cancellation of orders is possible. If, exceptionally, the purchase order (order) is cancelled by mutual consent without stipulation of a cancellation fee, the cancellation fee to be paid by the customer amounts to 20% or EURO 36.00 as basic sum plus value added tax. This stipulation does not entitle the customer to unilateral cancellation of a contract (order). Goods without defects may be returned to VWR International GmbH only with explicit consent and carriage paid. Refrigerated goods are generally excluded from returns.

4. Delivery Deadlines, Delay and Non-Delivery

We make an effort to keep the delivery deadlines defined by us, but since delivery does not only depend on us, we cannot make any binding promises. The delivery period shall be extended by force majeure or by all obstacles that by reasonable means cannot be influenced by us.

Exceeded delivery deadlines shall not entitle our customers to raise damage claims, but our customers may, if the delivery deadline has been exceeded by more than four weeks and this is exclusively due to our fault, withdraw from the contract by registered mail. This withdrawal shall become effective, if we fail to make the delivery within a reasonable extension period of at least four weeks. The customer shall not be entitled to raise any further claims.

5. Delivery, Exclusion of Indemnification

The goods shall be delivered in standard commercial quality. A reasonable amount of partial deliveries is admissible. In case of purchase according to specimen or sample the characteristics of the specimen or sample are not guaranteed. Any liability for the appropriateness of the delivered goods for the purpose intended by the customer is excluded as well as for damages caused by the product or its processing. In particular also our liability for material damages according to the product liability act is excluded. Our liability for damages resulting from consulting is excluded as well.
Customer complaints can generally only be considered when written orders were made.


6. Shipment and Transfer of Risk

Delivery shall be made ex works, freight charges shall be borne by the purchaser. Due to increased handling expenses a lump sum for small orders amounting to EURO 15.00 will be charged for orders below EURO 150.00. If the customer has special requests concerning the type of shipment, e.g. express shipment, the additional costs shall be charged.

The risk shall be transferred to the customer when the goods are transferred to a forwarder or carrier, in any case when they leave of the warehouse.

7. Use

The products we supply are intended for use in accordance with the documentation and manufacturer specifications under the user’s sole responsibility. Nevertheless, for some specific products (Active Pharmaceutical Ingredients), VWR, as a distributor, is not able to fulfill the requirements (European guidelines for GMP Medicinal products for human and veterinary use) and cannot sell the products.

The products we supply cannot be used as active ingredients, in human or veterinary medicine. For use as pharmaceutical, cosmetic, food additives, agricultural aids, pesticides or domestic, the customer must contact the VWR local sales organisation.

Our customers that intend to use these products for a pharmaceutical, cosmetic, food or any other use must carry out their own internal checks and controls to ensure that they are in compliance with their local laws,regulations (e.g. European pharmacopeia, etc.).

In no event VWR International shall be liable if a customer uses a product that does not fit with the intended use.

It is customer's responsibility to comply with all applicable health, safety and other regulations and to take appropriate steps in relation to the storage, handling, sale and use of the products.

8. Declaration of decontamination by the customer

Instruments or other materials, that are handed over to VWR, must be decontaminated by the customer or the end-user. Decontamination is being confirmed by a decontamination declaration, that is accompanying the shipment.

For damage of any kind, that is caused by missing cleaning and/or decontamination, the customer or end-user is liable in its intentity.

Every owner of an instrument is obligated to forward this information in case of sale or abandonment.

For return of reusable packaging point 8 is exclusively valid.

9. Refund system

Reusable packaging or containers (“reusable packaging”) remains property of the respective supplier and is only left to the customer as a loan.

VWR reserves the right to demand deposit fees for reusable packaging. The amount complies with the specifications of the respective supplier. The deposit fee will be refunded completely by return of undamaged, completely deflated and carriage paid return of the reusable packaging.

In case, that the reusable packaging ist not or damaged returned and/or has been diverted from its intended use by the customer, the customer expires his claim of refund of the deposit fee. Arising cleaning or disposal costs for VWR by reusable packaging, not being completely deflated or diverted from its intended use, will be at the customer´s expense.


10. Prices and Payment

Value added tax shall be added to the prices.

In case of a considerable increase of prices after conclusion of the contract we shall be entitled to raise the prices accordingly.
With orders below EUR 1,000.00 we reserve ourselves the right to refrain from a separate confirmation of the order.

The purchase price has to be paid without discount within 14 days after the date of the invoice.

We shall, however, be entitled to make the delivery only for cash in advance or cash on delivery without having to state reasons therefor.

If we accept bills of exchange or checks, we do so only on account of payment. Payment shall be considered as made only after definitive cashing of the check. Discount charges and interest shall be borne by the customer.

Bank transfer charges shall be borne by the customer.

If the customer is in arrears with regard to any contractual obligation towards us, all invoices shall become due immediately. In case of delayed payment interest amounting to the bank rate of interest for overdraft facility at the time, but in any case a minimum of 12% plus VAT shall be charged.

Objections regarding the goods does not entitle to withhold payment. The customer shall not be entitled to any compensation for other claims.


11. Reservation of title

All goods delivered by us remain our property until full payment has been made. If such goods which remain our property are processed by our customer, property shall be shared in proportion to the value shares at the time of processing.

12. Warranty

Regardless of the fact whether our customer is a business man or not, the legal warranty stipulations for mutual sales transactions shall be applied. Compensation for consequential damage caused by defects in delivered goods shall be excluded.

13. Place of Fulfilment, Venue

Place of fulfilment of all services is Vienna. In case of dispute arising from or in connection with our contractual relation or its cancellation the court of competent jurisdiction for Vienna 1 shall, at first instance, have exclusive competence.


VWR International GmbH
Graumanngasse 7
1150 Vienna
Telephone +43 1 97 002 - 0
Telefax +43 1 97 002 - 600
E-Mail: info.at@vwr.com
Internet: www.vwr.com

Vienna, May 2018

FN: 189903 w
Commercial Court Vienna
VAT reg.-number: ATU 48152305
DVR-No.: 1034731
ARA-No.: 2158
ERA-No.: 50478

Y-27632 dihydrochloride (2024)

FAQs

What is the function of ROCK inhibitor Y-27632? ›

The use of ROCK inhibitors contributes to maintaining or restructuring of these epithelial structures, that is, Y-27632 increases cell–cell interaction and adhesion. As shown in Fig. 2, Y-27632 treatment may be used as a multifunctional reagent throughout the process of stem cell research.

How to reconstitute Y-27632? ›

Aseptically add 3 mL of sterile water (ATCC 60-2450) or D-PBS (ATCC 30-2200) to the 10 mg vial of ROCK Inhibitor Y27632. Mix thoroughly by pipetting. Aliquot the stock solution in working volumes based on routine use.

What are the effects of rho associated protein kinase inhibitor Y-27632 on intraocular pressure and outflow facility? ›

conclusions. Administration of Y-27632 caused a reduction in IOP and an increase in the outflow facility.

How to make a 10mm ROCK inhibitor? ›

To make 10 mM Rock Inhibitor stock solution, dilute 1 mg Rock Inhibitor (FW 320.26) into 295 μL sterile water (see Note 1). Aliquot 20–50 μL/tube and store at −80 °C for up to 1 year. Aliquots can be stored up to 2 months at 4 °C. Rock Inhibitor working stock solution will be used at 1 μL to 1 mL final medium volume.

What does ROCK inhibitor do? ›

Rho-kinase inhibitors (rho-associated protein kinase inhibitor or ROCK inhibitor) are a series of compounds that target rho kinase (ROCK) and inhibit the ROCK pathway. Clinical trials have found that inhibition of the ROCK pathway contributes to the cardiovascular benefits of statin therapy.

What is the mode of action of ROCK inhibitor? ›

ROCK Inhibition Promotes Attachment Through an Increase in Cell Spreading and Cofilin Activation. ROCK activates LIMK through phosphorylation, leading to cofilin phosphorylation and inactivation, resulting in actin stabilization. ROCK is also known to regulate stress fiber formation through the phosphorylation of MLC.

What is the solubility of Y-27632? ›

Y-27632 (hydrochloride) is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). It is also soluble in water. The solubility of Y-27632 (hydrochloride) in DMSO and water is approximately 30 mg/ml and approximately 1 mg/ml in ethanol and DMF.

What does rho kinase do? ›

Rho-associated kinase (Rho-kinase/ROCK/ROK) is an effector of the small GTPase Rho and belongs to the AGC family of kinases. Rho-kinase has pleiotropic functions including the regulation of cellular contraction, motility, morphology, polarity, cell division, and gene expression.

What drugs are rho kinase inhibitors? ›

Commonly Used Rho Kinase Inhibitors

Two commonly used RKIs are Ripasudil (K-115) and Netarsudil(AR-13503). Ripasudil has been clinically approved to treat glaucoma in Japan. Side effects of Ripasudil include dose-dependent conjunctival hyperemia and non-dose dependent conjunctival hemorrhage.

What are the side effects of protein inhibitors? ›

Common side effects of protease inhibitors
  • Abdominal pain.
  • Nausea and vomiting.
  • Diarrhea.
  • Rash.
  • Cough.
  • Fatigue.
  • Kidney stones.
  • Redistribution of fat on your body (lipodystrophy).

What are the side effects of protein kinase inhibitors? ›

The protein kinase inhibitors all have some degree of hepatotoxicity and many have been linked to cases of clinically apparent liver injury which can be severe and even fatal.

What are the side effects of rho kinase inhibitors for glaucoma? ›

It has also been clinically approved to treat glaucoma, specifically in the United States, and its intraocular pressure lowering effect has been shown to increase with latanoprost. Side effects of Netarsudil include conjunctival hyperemia and corneal verticillata, instillation site pain, and conjunctival hemorrhages.

What is the working concentration of rock inhibitor Y-27632? ›

For most hESC applications, reconstitute Y-27632 (Molar mass: 338.3) to 1 mM stock concentration in sterile distilled water. Aliquot and store at -20°C for up to one year. Aliquots can be thawed and diluted to a working concentration of 10 μM for hESC applications.

Why is it important to use more than one iPSC cell line? ›

Therefore, to ensure that the exhibited phenotypes are not unique to a specific iPS cell line or a particular patient, it is important to evaluate several iPS cell lines generated from the same patient as well as those generated from different patients with the same disease.

How to thaw iPSC? ›

Remove frozen vial of iPSCs from liquid nitrogen storage and place in 37°C water bath until most, but not all, cells are thawed.

What is the function of an inhibitor? ›

Inhibitors are chemical or biological molecules that regulate chemical reactions by slowing down or blocking them from occurring. Inhibitors often work to slow or stop enzymes — proteins that catalyze reactions.

What is the function of corrosion inhibitor? ›

Corrosion Inhibitors are used to stop rusting and anodic corrosion of metals. This is generally done via the coating of the metal surface with a chromate layer. Oxygen scavengers can be used as CIs to react with dissolved oxygen in the environment and can help in preventing cathodic corrosion.

What is the mechanism of action of ROCK inhibitors? ›

The mechanism of ROCK inhibitors' ability to decrease IOP is through direct action on the trabecular meshwork and Schlemm's canal cells (increases permeability), which play a large role in the resistance of aqueous humor outflow.

What is the function of oxidation inhibitors? ›

Most mineral oil dielectric fluids contain an added oxidation inhibitor which is a chemical additive that acts as a preservative. The purpose of the inhibitor is to prevent oxygen from reacting with the oil, thus slowing the aging rate of the oil (and also of the solid insulation).

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