Recombinant Human IL-17A (Human Cell-expressed) Protein (2024)

FAQs

What cells express IL17A? ›

Subsequent studies showed that lymphocytes including CD4+, CD8+, gamma-delta T (γδ-T), invariant NKT and innate lymphoid cells (ILCs) are primary sources of IL-17A (Cua and Tato, 2010). Non-T cells, such as neutrophils, have also been reported to produce IL-17A under certain circ*mstances (Taylor et al., 2014).

The recombinant human IL17A consists of 132 amino acids and predicts a molecular mass of 15.1 KDa. It migrates as an approximately 16, 19, and 20 KDa band in SDS-PAGE under reducing conditions due to different glycosylation. The molecular weight of this protein is around 31.6kDa verified by SEC-MALS (Routinely tested).

Background: IL-2. Recombinant IL-2 is widely used in cell culture for the expansion of T cells. IL-2 is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1-3). Mature human IL-2 shares 56% and 66% amino acid (aa) sequence identity with mouse and rat IL-2, respectively.

IL-17 is mainly secreted by immune cells;^{10,11,12,13,14,15,16} however, intestinal epithelial and Paneth cells can also secrete IL-17 in response to injury and inflammation to regulate local microbial communities.

The targets of IL-17 include primarily epithelial, endothelial and other stromal cells such as fibroblasts, osteoblasts, chondrocytes, and synovial cells (21, 77, 78, 103, 137).

IL-17 was first discovered approximately 30 years ago. Members of this family have various biological functions, including driving an inflammatory cascade during infections and autoimmune diseases, as well as boosting protective immunity against various pathogens.

Previous studies have shown that IL-17 is a strong proinflammatory cytokine and that IL-17-producing autoreactive T cells play a major role in the pathogenesis of autoimmune diseases.

Posted April 24, 2020. Recombinant polypeptides, or proteins, are made using recombinant DNA techniques, while synthetic peptides are made by chemical peptide synthesis. Synthetic peptides have some advantages over recombinant proteins such as the ability to be generated as exact copies of protein fragments.

IL-17 exerts proatherogenic effects by inducing the production of cytokines, chemokines, and matrix metalloproteinases. IL-17 also promotes G-CSF-mediated granulopoiesis and the recruitment of immune cells.

What are recombinant proteins? Recombinant proteins are proteins encoded by recombinant DNA that has been cloned in an expression vector that supports expression of the gene and translation of messenger RNA. Modification of the gene by recombinant DNA technology can lead to expression of a mutant protein.

What two types of human proteins are made with bacterial recombinant DNA? ›

Recombinant DNA technology has been successfully applied to make important proteins used in the treatment of human diseases, such as insulin and growth hormone.

The first human hormone produced by recombinant DNA technology is insulin.

Interleukin-17-expressing cells are particularly abundant in the gut, including classic αβ-T cells as well as more innate cells such as γδ-T and natural killer T cells.

Interleukin 17 family (IL17 family) is a family of pro-inflammatory cystine knot cytokines. They are produced by a group of T helper cell known as T helper 17 cell in response to their stimulation with IL-23.

Cosentyx (secukinumab), Taltz (ixekizumab), and Siliq (brodalumab) are three very similar IL-17 inhibitors that work by blocking the inflammatory pathway of interleukin-17A. These biologic drugs are all FDA-approved for the treatment of psoriasis and psoriatic arthritis.

Th17 cells produce IL-17, IL-17F, and IL-22, thereby inducing a massive tissue reaction owing to the broad distribution of the IL-17 and IL-22 receptors. Th17 cells also secrete IL-21 to communicate with the cells of the immune system.

It is produced and secreted by a variety of cell types although the vast majority of studies have focussed on its production within cells of the innate immune system, such as monocytes and macrophages.

Although B cells express TLRs, B cells do not make IL-17 after stimulation with various TLR ligands, and B cells that lack MyD88, a key molecule in the TLR signal-transduction pathway, still produce IL-17 after infection with T. cruzi⁴.

Th17 cells are a subset of CD4⁺ effector T cells characterized by expression of the IL-17-family cytokines, IL-17A and IL-17F. Since their discovery nearly a decade ago, Th17 cells have been implicated in the regulation of dozens of immune-mediated inflammatory diseases and cancer.